General Cell Lines and Cell Cultures
Eurofins Discoverx PathHunter CHO-K1 cyno MRGPRX2 β-Arrestin Cell Line, 2 vials. (93-1134C2)
The PathHunter cyno MRGPRX2 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. PAMP-20) based activation of cyno MRGPRX2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged cyno MRGPRX2 and EA-tagged β-Arrestin-2. Activation of cyno MRGPRX2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Cynomolgus monkey version of the protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PrecisION hASIC3 Recombinant Stable Cell Line, 2 vials. (CYL3055)
The PrecisION hASIC3 Recombinant Stable Cell Line is a stable, engineered cell line for use in studying ASIC3 ion channels, including drug candidates which affect channel function. This cell line enables assessment of ion channel function via detection of electrophysiological signals. The included cell line overexpresses ASIC3 ion channels. Upon ligand/agonist induced channel activation, response can be detected via automated or manual electrophysiological techniques. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/ion-channels.
Discovery Life Sciences Ulcerative Colitis PBMC
PBMCs Peripheral Blood Mononuclear Cells are immune cells isolated from whole blood and viably cryopreserved providing a foundational material for immunology studies cell therapy research and functional immune assays
Biochain Institute Inc Matched Pair - Total Protein - Human Primary Tumor and Normal Tissue (PP): Kidney, 0.2 x2 mg /PK
BioChain's human total protein matched pair products include: Primary Pair (PP), Primary and Metastatic Pair (PM). PP consists of total protein isolated from primary tumor and its adjacent normal tissue; PM consists of total protein from primary tumor andcorresponding metastastic tumor. Total proteins in each pair are prepared from the same donor. This product line is designedfor identifying tumor-specific genes and tumor metastasis genes.
Eurofins Discoverx PathHunter CHO-K1 GPR12 β-Arrestin Orphan GPCR Cell Line, 2 vials. (93-0329C2A)
The PathHunter GPR12 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand based activation of GPR12 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR12 and EA-tagged β-Arrestin-2. Activation of GPR12 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. This is an orphan receptor without a known ligand. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Creative Bioarray CAL-120 cells, human breast adenocarcinoma, pleural effusion derived, triple-negative, heterogeneous, adherent cells, monolayer culture, 90% DMEM + 10% h.i. FBS
CAL-120 cells, human breast adenocarcinoma, pleural effusion derived, triple-negative, heterogeneous, adherent cells, monolayer culture, 90% DMEM + 10% h.i. FBS
Eurofins Discoverx ChemiScreen M4 Muscarinic Acetylcholine Receptor Stable Cell Line, 2 vials. (HTS117C)
The ChemiScreen M4 Muscarinic Acetylcholine Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. acetylcholine) based activation of CHRM4 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CHRM4 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of CHRM4, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx PathHunter PTAFR Activated GPCR Internalization U2OS Cell Line, 2 vials. (93-0680C3)
The PathHunter PTAFR Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line uses EFC technology to measure ligand-induced internalization of PTAFR via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged PTAFR. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting PTAFR-arrestin complex is internalized to the early endosome, leading to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CHO-K1 GLP2R β-Arrestin Cell Line, 2 vials. (93-0572C2)
The PathHunter GLP2R cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. GLP-2) based activation of GLP2R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GLP2R and EA-tagged β-Arrestin-2. Activation of GLP2R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CHO-K1 mGLP1R β-Arrestin-1 Cell Line, 2 vials. (93-0974C2)
The PathHunter mGLP1R (ARR1) cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. exendin-4) based activation of mGLP1R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mGLP1R and EA-tagged β-Arrestin-1. Activation of mGLP1R stimulates the recruitment of β-Arrestin-1 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter HEK 293 cyno CXCR1 β-Arrestin Cell Line, 2 vials. (93-0980C1)
The PathHunter cyno CXCR1 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. CXCL8/IL8) based activation of CXCR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged cyno CXCR1 and EA-tagged β-Arrestin-2. Activation of CXCR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Cynomolgus monkey version of this protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Biochain Institute Inc Paraffin Tissue Section - Human Colon Tumor: Non-hodgkin's lymphoma, 5 sides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's formalin fixed paraffin embedded (FFPE) tissue section line encompasses a large selection of tissue sections which are suitable for immunohistochemistry and in situ hybridization assays. The tissue sections are approximately 5 um thick. Each slide contains tissue from a single human tumor. Documentation of tissues' clinical histories may be available upon request. For those customers who want to perform FFPE DNA and RNA isolation, BioChain can provide tissue section slices in tubes instead of mounting them on glass slides.
Montana Molecular Human Succinate Receptor (SUCNR1, SUCR1) BacMam, 30mL
Use this assay to express the human Succinate receptor in your cells of interest. BacMam packaging provides efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in primary or iPSC-derived cells. Importantly, BacMam expression is easily titrateable to suit your experimental needs. This tool can be co-expressed with Montana Molecular's assays for cAMP to investigate signaling dynamics at the SUCNR1 receptor.